IRIFIV Research

Evaluation of Oxidative and Nitrosative Stress Markers Related to Inflammation in the Cumulus Cells and Follicular Fluid of Women Undergoing Intracytoplasmic Sperm Injection: A Prospective Study

A Fertility Research Publication from IRIFIV – Centre de Fertilité Casablanca, Maroc

Authors

Noureddine Louanjli, Hasnae Debbarh, Ph.D. Malak Jamil, Ph.D., Hasnae Jelloul, M.Sc., Amal Kabit, M.Sc., Mohamed Ennaji, M.Sc. Noureddine Louanjli, M.D., Rachida Cadi, Ph.D.

Journal / Date

International Journal of Fertility & Sterility

Introduction & Clinical Relevance:

Intracytoplasmic sperm injection (ICSI) is a widely used assisted reproductive technology, yet its success remains strongly influenced by the quality of the oocyte microenvironment. Oxidative and nitrosative stress, characterized by excessive production of reactive oxygen species (ROS) and reactive nitrogen species (RNS), plays a critical role in inflammation, oocyte competence, embryo development, and pregnancy outcomes.This prospective scientific study involving IRIFIV Casablanca evaluates key oxidative and nitrosative stress markers in cumulus cells and follicular fluid of women undergoing ICSI. The findings demonstrate that elevated levels of lipid peroxidation, protein oxidation, nitric oxide, peroxynitrite, and inflammatory markers are significantly associated with advanced maternal age, reduced oocyte maturity, poor embryo quality, lower oocyte yield, and negative pregnancy outcomes. These results highlight the importance of the follicular microenvironment as a determinant of reproductive success.At IRIFIV Centre de Fertilité Casablanca (Maroc), this research supports the integration of molecular and inflammatory biomarkers into fertility assessment and IVF decision-making. By identifying patients at risk of suboptimal ICSI outcomes, IRIFIV enhances personalized treatment strategies, optimizes ovarian stimulation protocols, and reinforces its position as a leading fertility and IVF center in Morocco and internationally.

Abstract:

Oxidative/nitrosative stress in the oocyte microenvironment could have an impact on intracytoplasmic sperm injection (ICSI) outcomes. The presence of reactive oxygen species (ROS) and reactive nitrogen species (RNS) can stimulate the secretion of pro-inflammatory cytokines, leading to chronic inflammation and potentially affecting embryo as well as oocyte quality. This study aimed to examine the relationship of lipid peroxidation [measured by the malondialdehyde (MDA) assay] with protein carbonyl [measured by the 2,4 dinitrophenylhydrazine (DNPH) assay] levels in cumulus cells (CCs), as well as nitric oxide (NO), peroxynitrite (ONOO-), and C-reactive protein (CRP) levels in follicular fluid (FF). The potential relationship of these levels with ICSI outcomes was also evaluated. Materials and Methods: In this prospective study, 63 FF samples and their corresponding CCs were collected for ICSI procedures. Spectrophotometry was used to assess levels of DNPH, MDA, NO, and ONOO-. CRP levels were evaluated using an immunoturbidimetric assay. Results: The patients under 37 years with normal ovarian reserve had significantly lower levels of MDA, DNPH, NO, ONOO-, and CRP compared to those over 37 years. Additionally, we observed higher levels of MDA, DNPH, NO, ONOO-, and CRP in the group with an oocyte maturity rate of less than 60%. No significant difference was observed between the DNPH levels and factors such as infertility duration, embryo quality, pregnancy, or the number of retrieved oocytes. A higher level of MDA, NO, ONOO-, and CRP was found to be significantly related to the lower number of retrieved oocytes, longer periods of infertility, poor embryo quality, and negative pregnancy outcomes. Conclusion: Oxidative/nitrosative stress, linking to inflammation in the oocyte microenvironment, can be considered as a potentially useful biomarker for assessing the development and competence of oocytes and embryos and predicting ICSI outcomes.

Keywords:

oxidative stress ICSI, nitrosative stress fertility, cumulus cells biomarkers, follicular fluid inflammation, ICSI outcomes, oocyte quality, female infertility

IRIFIV Research

Evaluation of Oxidative and Nitrosative Stress Markers Related to Inflammation in the Cumulus Cells and Follicular Fluid of Women Undergoing Intracytoplasmic Sperm Injection: A Prospective Study

A Fertility Research Publication from IRIFIV – Centre de Fertilité Casablanca, Maroc

Authors

Noureddine Louanjli, Hasnae Debbarh, Ph.D. Malak Jamil, Ph.D., Hasnae Jelloul, M.Sc., Amal Kabit, M.Sc., Mohamed Ennaji, M.Sc. Noureddine Louanjli, M.D., Rachida Cadi, Ph.D.

Journal / Date

International Journal of Fertility & Sterility

Introduction & Clinical Relevance:

Intracytoplasmic sperm injection (ICSI) is a widely used assisted reproductive technology, yet its success remains strongly influenced by the quality of the oocyte microenvironment. Oxidative and nitrosative stress, characterized by excessive production of reactive oxygen species (ROS) and reactive nitrogen species (RNS), plays a critical role in inflammation, oocyte competence, embryo development, and pregnancy outcomes.This prospective scientific study involving IRIFIV Casablanca evaluates key oxidative and nitrosative stress markers in cumulus cells and follicular fluid of women undergoing ICSI. The findings demonstrate that elevated levels of lipid peroxidation, protein oxidation, nitric oxide, peroxynitrite, and inflammatory markers are significantly associated with advanced maternal age, reduced oocyte maturity, poor embryo quality, lower oocyte yield, and negative pregnancy outcomes. These results highlight the importance of the follicular microenvironment as a determinant of reproductive success.At IRIFIV Centre de Fertilité Casablanca (Maroc), this research supports the integration of molecular and inflammatory biomarkers into fertility assessment and IVF decision-making. By identifying patients at risk of suboptimal ICSI outcomes, IRIFIV enhances personalized treatment strategies, optimizes ovarian stimulation protocols, and reinforces its position as a leading fertility and IVF center in Morocco and internationally.

Abstract:

Oxidative/nitrosative stress in the oocyte microenvironment could have an impact on intracytoplasmic sperm injection (ICSI) outcomes. The presence of reactive oxygen species (ROS) and reactive nitrogen species (RNS) can stimulate the secretion of pro-inflammatory cytokines, leading to chronic inflammation and potentially affecting embryo as well as oocyte quality. This study aimed to examine the relationship of lipid peroxidation [measured by the malondialdehyde (MDA) assay] with protein carbonyl [measured by the 2,4 dinitrophenylhydrazine (DNPH) assay] levels in cumulus cells (CCs), as well as nitric oxide (NO), peroxynitrite (ONOO-), and C-reactive protein (CRP) levels in follicular fluid (FF). The potential relationship of these levels with ICSI outcomes was also evaluated. Materials and Methods: In this prospective study, 63 FF samples and their corresponding CCs were collected for ICSI procedures. Spectrophotometry was used to assess levels of DNPH, MDA, NO, and ONOO-. CRP levels were evaluated using an immunoturbidimetric assay. Results: The patients under 37 years with normal ovarian reserve had significantly lower levels of MDA, DNPH, NO, ONOO-, and CRP compared to those over 37 years. Additionally, we observed higher levels of MDA, DNPH, NO, ONOO-, and CRP in the group with an oocyte maturity rate of less than 60%. No significant difference was observed between the DNPH levels and factors such as infertility duration, embryo quality, pregnancy, or the number of retrieved oocytes. A higher level of MDA, NO, ONOO-, and CRP was found to be significantly related to the lower number of retrieved oocytes, longer periods of infertility, poor embryo quality, and negative pregnancy outcomes. Conclusion: Oxidative/nitrosative stress, linking to inflammation in the oocyte microenvironment, can be considered as a potentially useful biomarker for assessing the development and competence of oocytes and embryos and predicting ICSI outcomes.

Keywords:

oxidative stress ICSI, nitrosative stress fertility, cumulus cells biomarkers, follicular fluid inflammation, ICSI outcomes, oocyte quality, female infertility

Le Département de Recherche Scientifique d’IRIFIV Centre de Fertilité à Casablanca (Maroc) est dédié à l’avancement de la médecine de la reproduction, de la FIV et des approches diagnostiques innovantes. Les travaux de recherche menés par l’équipe IRIFIV contribuent au développement de nouvelles stratégies cliniques, biologiques et génétiques visant à améliorer la prise en charge de l’infertilité et des pathologies gynécologiques complexes.

À propos du département de recherche scientifique de l’IRIFIV

Le Département de Recherche Scientifique d’IRIFIV Centre de Fertilité à Casablanca (Maroc) est dédié à l’avancement de la médecine de la reproduction, de la FIV et des approches diagnostiques innovantes. Les travaux de recherche menés par l’équipe IRIFIV contribuent au développement de nouvelles stratégies cliniques, biologiques et génétiques visant à améliorer la prise en charge de l’infertilité et des pathologies gynécologiques complexes.

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Fondé en 1992, avec plus de 34 ans d’expérience et des milliers de bébés nés, IRIFIV Casablanca est le premier centre structuré marocain de procréation médicalement assistée et l’un des centres de fertilité et FIV les plus reconnus à Casablanca et au Maroc. La clinique est spécialisée dans tous les cas d’infertilité, y compris les cas complexes. Nous utilisons des technologies et équipements de dernière génération, dont certains uniques au Maroc. IRIFIV et le laboratoire Labomac constituent un terrain de stage pratique en FIV dans le cadre du Diplôme Universitaire de Biologie et Génétique de la Reproduction de l’Université Mohammed VI des Sciences de la Santé, et sont actifs en recherche, formation médicale et collaborations internationales.

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IRIFIV is a multidisciplinary fertility center in Casablanca, Morocco, specializing in IVF and ICSI. Since 1992, over 32 years of expertise and thousands of successful births demonstrate our commitment. We offer all techniques to evaluate and treat infertility with a personalized approach, multidisciplinary team, latest technology, complementary therapies, scientific research, teaching, professional development, and international collaboration.

Le 1er Centre multidisciplinaire structuré d’Assistance Médicale à la Procréation (AMP) au Maroc

Fondé en 1992, avec plus de 34 ans d’expérience et des milliers de bébés nés, IRIFIV Casablanca est le premier centre structuré marocain de procréation médicalement assistée et l’un des centres de fertilité et FIV les plus reconnus à Casablanca et au Maroc. La clinique est spécialisée dans tous les cas d’infertilité, y compris les cas complexes. Nous utilisons des technologies et équipements de dernière génération, dont certains uniques au Maroc. IRIFIV et le laboratoire Labomac constituent un terrain de stage pratique en FIV dans le cadre du Diplôme Universitaire de Biologie et Génétique de la Reproduction de l’Université Mohammed VI des Sciences de la Santé, et sont actifs en recherche, formation médicale et collaborations internationales.

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IRIFIV Research

Evaluation of Oxidative and Nitrosative Stress Markers Related to Inflammation in the Cumulus Cells and Follicular Fluid of Women Undergoing Intracytoplasmic Sperm Injection: A Prospective Study

A Fertility Research Publication from IRIFIV – Centre de Fertilité Casablanca, Maroc

Authors

Noureddine Louanjli, Hasnae Debbarh, Ph.D. Malak Jamil, Ph.D., Hasnae Jelloul, M.Sc., Amal Kabit, M.Sc., Mohamed Ennaji, M.Sc. Noureddine Louanjli, M.D., Rachida Cadi, Ph.D.

Journal / Date

International Journal of Fertility & Sterility

04/01/2025

Introduction & Clinical Relevance:

Abstract:

Keywords:

oxidative stress ICSI, nitrosative stress fertility, cumulus cells biomarkers, follicular fluid inflammation, ICSI outcomes, oocyte quality, female infertility

IRIFIV Research

Evaluation of Oxidative and Nitrosative Stress Markers Related to Inflammation in the Cumulus Cells and Follicular Fluid of Women Undergoing Intracytoplasmic Sperm Injection: A Prospective Study

A Fertility Research Publication from IRIFIV – Centre de Fertilité Casablanca, Maroc

Authors

Noureddine Louanjli, Hasnae Debbarh, Ph.D. Malak Jamil, Ph.D., Hasnae Jelloul, M.Sc., Amal Kabit, M.Sc., Mohamed Ennaji, M.Sc. Noureddine Louanjli, M.D., Rachida Cadi, Ph.D.

Journal / Date

International Journal of Fertility & Sterility

04/01/2025

Introduction & Clinical Relevance:

Abstract:

Keywords:

oxidative stress ICSI, nitrosative stress fertility, cumulus cells biomarkers, follicular fluid inflammation, ICSI outcomes, oocyte quality, female infertility

À propos du département de recherche scientifique de l’IRIFIV

À propos du département de recherche scientifique de l’IRIFIV

Vous aimerez aussi

IRIFIV Research

Session 70: Genetics: what genomes gone wrong can tell us

IRIFIV Research

Protéome du liquide folliculaire et de l’ovocyte Proteomics of follicular fluid and oocyte

IRIFIV Research

PBMC-autologous immunotherapy for repeated implantation failures in IVF

prêt à démarrer votre projet parental?

prêt à démarrer votre projet parental ?

Nos Services

À PROPOS

RESOURCES

PATIENTS INTERNATIONAUX

ARTICLES RÉCENTS

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+212 5 22 94 97 72

+212 5 22 94 97 72