IRIFIV Research

Clinical Guidelines for Sperm DNA Fragmentation (SDF) is Associated with Male Infertility from Reactive Oxygen Species (ROS)

A Fertility Research Publication from IRIFIV – Centre de Fertilité Casablanca, Maroc

Authors

Noureddine Louanjli, Mustafa Zakaria, Aya Al-ibraheemi, Mohamed Ennaji, Wassym R. Senhaji, Abdelhafid Natiq, Romaissa Boutiche, Modou M. Mbaye, Mohamed Zarqaoui, Muhjah F. Hassan, Hayder A. Mossa and Noureddine Louanjli

Journal / Date

Gynecology & Reproductive Health

Introduction & Clinical Relevance:

This pivotal scientific review from IRIFIV Fertility Center in Casablanca, Morocco, tackles a fundamental yet complex cause of male infertility: Sperm DNA Fragmentation (SDF). Co-authored by leading authority Dr. Noureddine Louanjli, the paper establishes clear clinical guidelines for understanding how damage to sperm genetic material, often caused by Reactive Oxygen Species (ROS), impacts fertility and Assisted Reproductive Technology (ART) outcomes.
The clinical relevance of this work is immense for couples struggling to conceive. High SDF levels are linked to lower fertilization rates, poor embryo quality, increased miscarriage risk, and reduced success with IVF and ICSI treatments. This publication serves as a crucial resource for both clinicians and patients, outlining advanced diagnostic tools (like TUNEL assay) and effective management strategies, including lifestyle modifications and targeted treatments. By providing evidence-based guidelines, IRIFIV reinforces its role as a center of excellence that addresses all facets of infertility, including advanced male fertility diagnosis and treatment in Morocco, to improve overall chances of achieving a healthy pregnancy.

Abstract:

Sperm DNA fragmentation is common in infertile male. Besides, sperm DNA integrity is essential for fertilization and healthy offspring development. Numerous genetic and environmental elements are associated with impacting sperm DNA integrity negatively. Such as lifestyle, ageing, industrial toxins, and infection. The mechanisms behind SDF are many, but apoptosis and reactive oxygen species are considered the main SDF mechanisms. The management of male infertility has led to the desire for more advanced SDF diagnostic tools to diagnose sperm DNA. Numerous sperm DNA damage assays such as terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labelling (TUNEL) assay and in situ nick translation (ISNT) are available to enhance SDF diagnosing and ultimately to better SDF management. Clinical SDF can lead to a low pregnancy rate, defects in embryo development and impaired offspring health. Moreover, SDF can impact the effectiveness of assisted reproductive technology through transfer genetics impartment to the embryo b in vitro fertilization or intracytoplasmic sperm injection. SDF can be mange through lifestyle changing, treating existing infection in the male reproductive tract and reactive oxygen species.

Keywords:

Sperm DNA Fragmentation SDF, male infertility causes, reactive oxygen species ROS, sperm DNA testing, TUNEL assay, male fertility treatment, IVF success, clinical guidelines, IRIFIV Casablanca, Dr. Noureddine Louanjli, oxidative stress, semen analysis

IRIFIV Research

Clinical Guidelines for Sperm DNA Fragmentation (SDF) is Associated with Male Infertility from Reactive Oxygen Species (ROS)

A Fertility Research Publication from IRIFIV – Centre de Fertilité Casablanca, Maroc

Authors

Noureddine Louanjli, Mustafa Zakaria, Aya Al-ibraheemi, Mohamed Ennaji, Wassym R. Senhaji, Abdelhafid Natiq, Romaissa Boutiche, Modou M. Mbaye, Mohamed Zarqaoui, Muhjah F. Hassan, Hayder A. Mossa and Noureddine Louanjli

Journal / Date

Gynecology & Reproductive Health

Introduction & Clinical Relevance:

This pivotal scientific review from IRIFIV Fertility Center in Casablanca, Morocco, tackles a fundamental yet complex cause of male infertility: Sperm DNA Fragmentation (SDF). Co-authored by leading authority Dr. Noureddine Louanjli, the paper establishes clear clinical guidelines for understanding how damage to sperm genetic material, often caused by Reactive Oxygen Species (ROS), impacts fertility and Assisted Reproductive Technology (ART) outcomes.
The clinical relevance of this work is immense for couples struggling to conceive. High SDF levels are linked to lower fertilization rates, poor embryo quality, increased miscarriage risk, and reduced success with IVF and ICSI treatments. This publication serves as a crucial resource for both clinicians and patients, outlining advanced diagnostic tools (like TUNEL assay) and effective management strategies, including lifestyle modifications and targeted treatments. By providing evidence-based guidelines, IRIFIV reinforces its role as a center of excellence that addresses all facets of infertility, including advanced male fertility diagnosis and treatment in Morocco, to improve overall chances of achieving a healthy pregnancy.

Abstract:

Sperm DNA fragmentation is common in infertile male. Besides, sperm DNA integrity is essential for fertilization and healthy offspring development. Numerous genetic and environmental elements are associated with impacting sperm DNA integrity negatively. Such as lifestyle, ageing, industrial toxins, and infection. The mechanisms behind SDF are many, but apoptosis and reactive oxygen species are considered the main SDF mechanisms. The management of male infertility has led to the desire for more advanced SDF diagnostic tools to diagnose sperm DNA. Numerous sperm DNA damage assays such as terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labelling (TUNEL) assay and in situ nick translation (ISNT) are available to enhance SDF diagnosing and ultimately to better SDF management. Clinical SDF can lead to a low pregnancy rate, defects in embryo development and impaired offspring health. Moreover, SDF can impact the effectiveness of assisted reproductive technology through transfer genetics impartment to the embryo b in vitro fertilization or intracytoplasmic sperm injection. SDF can be mange through lifestyle changing, treating existing infection in the male reproductive tract and reactive oxygen species.

Keywords:

Sperm DNA Fragmentation SDF, male infertility causes, reactive oxygen species ROS, sperm DNA testing, TUNEL assay, male fertility treatment, IVF success, clinical guidelines, IRIFIV Casablanca, Dr. Noureddine Louanjli, oxidative stress, semen analysis

Le Département de Recherche Scientifique d’IRIFIV Centre de Fertilité à Casablanca (Maroc) est dédié à l’avancement de la médecine de la reproduction, de la FIV et des approches diagnostiques innovantes. Les travaux de recherche menés par l’équipe IRIFIV contribuent au développement de nouvelles stratégies cliniques, biologiques et génétiques visant à améliorer la prise en charge de l’infertilité et des pathologies gynécologiques complexes.

À propos du département de recherche scientifique de l’IRIFIV

Le Département de Recherche Scientifique d’IRIFIV Centre de Fertilité à Casablanca (Maroc) est dédié à l’avancement de la médecine de la reproduction, de la FIV et des approches diagnostiques innovantes. Les travaux de recherche menés par l’équipe IRIFIV contribuent au développement de nouvelles stratégies cliniques, biologiques et génétiques visant à améliorer la prise en charge de l’infertilité et des pathologies gynécologiques complexes.

Vous aimerez aussi

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12/31/2025
Acomprehensive review about sperm-oocyte interactions and key activation factors of fertilization in mammals
Soukaina Azil, Yassmine Louanjli, Noureddine Louanjli, Moncef Benkhalifa, Bouchra Ghazi
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12/30/2025
Epidemiological, Clinical, and Biomarker Profile of Male Infertility in Morocco: A Retrospective Single-Center Study of 1399 Cases
Henri Hubert Kwizera Tsinda, Modou Mamoune Mbaye, Loïc Koumba, Reine Rolande Ada Edou, Achraf Zakaria, Noureddine Louanjli, Bouchra Ghazi, Fatima Maachi, Hakima
IRIFIV Research
07/14/2009
Mutations in the protamine locus: Association with spermatogenic failure?
L. Imken, H. Rouba, B. El Houate, N. Louanjli, A. Barakat, A. Chafik, K. McElreavey

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Fondé en 1992, avec plus de 34 ans d’expérience et des milliers de bébés nés, IRIFIV Casablanca est le premier centre structuré marocain de procréation médicalement assistée et l’un des centres de fertilité et FIV les plus reconnus à Casablanca et au Maroc. La clinique est spécialisée dans tous les cas d’infertilité, y compris les cas complexes. Nous utilisons des technologies et équipements de dernière génération, dont certains uniques au Maroc. IRIFIV et le laboratoire Labomac constituent un terrain de stage pratique en FIV dans le cadre du Diplôme Universitaire de Biologie et Génétique de la Reproduction de l’Université Mohammed VI des Sciences de la Santé, et sont actifs en recherche, formation médicale et collaborations internationales.

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Le 1er Centre multidisciplinaire structuré d’Assistance Médicale à la Procréation (AMP) au Maroc

Fondé en 1992, avec plus de 34 ans d’expérience et des milliers de bébés nés, IRIFIV Casablanca est le premier centre structuré marocain de procréation médicalement assistée et l’un des centres de fertilité et FIV les plus reconnus à Casablanca et au Maroc. La clinique est spécialisée dans tous les cas d’infertilité, y compris les cas complexes. Nous utilisons des technologies et équipements de dernière génération, dont certains uniques au Maroc. IRIFIV et le laboratoire Labomac constituent un terrain de stage pratique en FIV dans le cadre du Diplôme Universitaire de Biologie et Génétique de la Reproduction de l’Université Mohammed VI des Sciences de la Santé, et sont actifs en recherche, formation médicale et collaborations internationales.

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IRIFIV Research

Clinical Guidelines for Sperm DNA Fragmentation (SDF) is Associated with Male Infertility from Reactive Oxygen Species (ROS)

A Fertility Research Publication from IRIFIV – Centre de Fertilité Casablanca, Maroc

Authors

Noureddine Louanjli, Mustafa Zakaria, Aya Al-ibraheemi, Mohamed Ennaji, Wassym R. Senhaji, Abdelhafid Natiq, Romaissa Boutiche, Modou M. Mbaye, Mohamed Zarqaoui, Muhjah F. Hassan, Hayder A. Mossa and Noureddine Louanjli

Journal / Date

Gynecology & Reproductive Health

07/15/2021

Introduction & Clinical Relevance:

Abstract:

Keywords:

Sperm DNA Fragmentation SDF, male infertility causes, reactive oxygen species ROS, sperm DNA testing, TUNEL assay, male fertility treatment, IVF success, clinical guidelines, IRIFIV Casablanca, Dr. Noureddine Louanjli, oxidative stress, semen analysis

IRIFIV Research

Clinical Guidelines for Sperm DNA Fragmentation (SDF) is Associated with Male Infertility from Reactive Oxygen Species (ROS)

A Fertility Research Publication from IRIFIV – Centre de Fertilité Casablanca, Maroc

Authors

Noureddine Louanjli, Mustafa Zakaria, Aya Al-ibraheemi, Mohamed Ennaji, Wassym R. Senhaji, Abdelhafid Natiq, Romaissa Boutiche, Modou M. Mbaye, Mohamed Zarqaoui, Muhjah F. Hassan, Hayder A. Mossa and Noureddine Louanjli

Journal / Date

Gynecology & Reproductive Health

07/15/2021

Introduction & Clinical Relevance:

Abstract:

Keywords:

Sperm DNA Fragmentation SDF, male infertility causes, reactive oxygen species ROS, sperm DNA testing, TUNEL assay, male fertility treatment, IVF success, clinical guidelines, IRIFIV Casablanca, Dr. Noureddine Louanjli, oxidative stress, semen analysis

À propos du département de recherche scientifique de l’IRIFIV

À propos du département de recherche scientifique de l’IRIFIV

Vous aimerez aussi

IRIFIV Research

Acomprehensive review about sperm-oocyte interactions and key activation factors of fertilization in mammals

IRIFIV Research

Epidemiological, Clinical, and Biomarker Profile of Male Infertility in Morocco: A Retrospective Single-Center Study of 1399 Cases

IRIFIV Research

Mutations in the protamine locus: Association with spermatogenic failure?

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PATIENTS INTERNATIONAUX

ARTICLES RÉCENTS

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+212 5 22 94 97 72

+212 5 22 94 97 72