IRIFIV Research

Evaluation of In vitro Vitamin D Supplementation and its Impact on Sperm Quality: A Controlled Experimental Study

A Fertility Research Publication from IRIFIV – Centre de Fertilité Casablanca, Maroc

Authors

Noureddine Louanjli, Chaimaa Hilali, Ez-Zahidy Douae, Chafika Nadifi, Houda Benrahma, Nouama Bouanani, Kettani Halabi Mohamed, Moncef Benkhalifa, Noureddine Louanjli, Fatima Azzahra Lahlou

Journal / Date

Journal of Human Reproductive Sciences

Introduction & Clinical Relevance:

Male infertility remains a significant global health concern, with sperm quality playing a decisive role in natural conception and assisted reproductive outcomes. Beyond its established role in bone metabolism and immune regulation, Vitamin D has recently attracted attention for its potential influence on male reproductive function. This controlled in vitro study evaluates the impact of Vitamin D supplementation on key sperm quality parameters, including motility, viability, sperm DNA fragmentation index, and chromatin decondensation. By analysing both normozoospermic and asthenozoospermic samples under standardized laboratory conditions, this research provides valuable insights into the dose-dependent effects of Vitamin D on sperm performance and DNA integrity. The observed improvements in sperm motility and DNA quality suggest that Vitamin D supplementation could represent a promising complementary approach in the management of male infertility, particularly within assisted reproduction settings such as IVF and ICSI. These findings further reinforce IRIFIV’s commitment to integrating scientifically validated laboratory strategies into comprehensive male fertility care.

Abstract:

Vitamin D, widely recognized for its role in bone health and immune function, has recently garnered significant interest for its potential impact on male reproductive health. Several studies have suggested that adequate Vitamin D levels may be associated with improved sperm quality and, consequently, enhanced male fertility. Aim The study was conducted to evaluate the effects of different concentrations of Vitamin D on sperm parameters in vitro, including motility, viability, DNA fragmentation index (DFI) and sperm DNA decondensation index. Settings and Design This is a prospective in vitro study conducted on 100 semen samples, comprising 50 normozoospermic and 50 asthenozoospermic samples. Materials and Methods Sperm samples were incubated with different concentrations of Vitamin D (1.625 × 10 ⁻¹ M; 1.625 × 10 ⁻² M; 1.625 × 10 ⁻³ M to 1.625 × 10 ⁻⁴ M) for varying durations (5, 15 and 30 min). Following incubation, sperm motility, viability, DFI and sperm DNA decondensation index were assessed using standardized laboratory protocols. Statistical Analysis Used Statistical analysis was performed using SPSS (version 27.0.1) with analysis of variance. Results were expressed as mean ± standard deviation, and P < 0.05 was considered significant. Results A significant improvement in sperm motility was observed at a concentration of 1.625 × 10 ⁻³ M in both normozoospermic and asthenozoospermic samples. No significant changes were noted in sperm viability across all tested concentrations. DFI and DNA decondensation index showed statistically significant improvements (P < 0.001) in both groups. Conclusion Vitamin D supplementation in vitro demonstrated a positive effect on certain sperm quality parameters, particularly motility and DNA integrity. These findings indicate that Vitamin D could represent a promising approach for enhancing male fertility, although further studies on larger cohorts are warranted to confirm these results and establish clinical protocols.

Keywords:

Vitamin D, male infertility, sperm quality, sperm motility, sperm DNA fragmentation, in vitro study, asthenozoospermia, assisted reproduction, IVF male factor

IRIFIV Research

Evaluation of In vitro Vitamin D Supplementation and its Impact on Sperm Quality: A Controlled Experimental Study

A Fertility Research Publication from IRIFIV – Centre de Fertilité Casablanca, Maroc

Authors

Noureddine Louanjli, Chaimaa Hilali, Ez-Zahidy Douae, Chafika Nadifi, Houda Benrahma, Nouama Bouanani, Kettani Halabi Mohamed, Moncef Benkhalifa, Noureddine Louanjli, Fatima Azzahra Lahlou

Journal / Date

Journal of Human Reproductive Sciences

Introduction & Clinical Relevance:

Male infertility remains a significant global health concern, with sperm quality playing a decisive role in natural conception and assisted reproductive outcomes. Beyond its established role in bone metabolism and immune regulation, Vitamin D has recently attracted attention for its potential influence on male reproductive function. This controlled in vitro study evaluates the impact of Vitamin D supplementation on key sperm quality parameters, including motility, viability, sperm DNA fragmentation index, and chromatin decondensation. By analysing both normozoospermic and asthenozoospermic samples under standardized laboratory conditions, this research provides valuable insights into the dose-dependent effects of Vitamin D on sperm performance and DNA integrity. The observed improvements in sperm motility and DNA quality suggest that Vitamin D supplementation could represent a promising complementary approach in the management of male infertility, particularly within assisted reproduction settings such as IVF and ICSI. These findings further reinforce IRIFIV’s commitment to integrating scientifically validated laboratory strategies into comprehensive male fertility care.

Abstract:

Vitamin D, widely recognized for its role in bone health and immune function, has recently garnered significant interest for its potential impact on male reproductive health. Several studies have suggested that adequate Vitamin D levels may be associated with improved sperm quality and, consequently, enhanced male fertility. Aim The study was conducted to evaluate the effects of different concentrations of Vitamin D on sperm parameters in vitro, including motility, viability, DNA fragmentation index (DFI) and sperm DNA decondensation index. Settings and Design This is a prospective in vitro study conducted on 100 semen samples, comprising 50 normozoospermic and 50 asthenozoospermic samples. Materials and Methods Sperm samples were incubated with different concentrations of Vitamin D (1.625 × 10 ⁻¹ M; 1.625 × 10 ⁻² M; 1.625 × 10 ⁻³ M to 1.625 × 10 ⁻⁴ M) for varying durations (5, 15 and 30 min). Following incubation, sperm motility, viability, DFI and sperm DNA decondensation index were assessed using standardized laboratory protocols. Statistical Analysis Used Statistical analysis was performed using SPSS (version 27.0.1) with analysis of variance. Results were expressed as mean ± standard deviation, and P < 0.05 was considered significant. Results A significant improvement in sperm motility was observed at a concentration of 1.625 × 10 ⁻³ M in both normozoospermic and asthenozoospermic samples. No significant changes were noted in sperm viability across all tested concentrations. DFI and DNA decondensation index showed statistically significant improvements (P < 0.001) in both groups. Conclusion Vitamin D supplementation in vitro demonstrated a positive effect on certain sperm quality parameters, particularly motility and DNA integrity. These findings indicate that Vitamin D could represent a promising approach for enhancing male fertility, although further studies on larger cohorts are warranted to confirm these results and establish clinical protocols.

Keywords:

Vitamin D, male infertility, sperm quality, sperm motility, sperm DNA fragmentation, in vitro study, asthenozoospermia, assisted reproduction, IVF male factor

Le Département de Recherche Scientifique d’IRIFIV Centre de Fertilité à Casablanca (Maroc) est dédié à l’avancement de la médecine de la reproduction, de la FIV et des approches diagnostiques innovantes. Les travaux de recherche menés par l’équipe IRIFIV contribuent au développement de nouvelles stratégies cliniques, biologiques et génétiques visant à améliorer la prise en charge de l’infertilité et des pathologies gynécologiques complexes.

À propos du département de recherche scientifique de l’IRIFIV

Le Département de Recherche Scientifique d’IRIFIV Centre de Fertilité à Casablanca (Maroc) est dédié à l’avancement de la médecine de la reproduction, de la FIV et des approches diagnostiques innovantes. Les travaux de recherche menés par l’équipe IRIFIV contribuent au développement de nouvelles stratégies cliniques, biologiques et génétiques visant à améliorer la prise en charge de l’infertilité et des pathologies gynécologiques complexes.

Vous aimerez aussi

IRIFIV Research
12/31/2025
Acomprehensive review about sperm-oocyte interactions and key activation factors of fertilization in mammals
Soukaina Azil, Yassmine Louanjli, Noureddine Louanjli, Moncef Benkhalifa, Bouchra Ghazi
IRIFIV Research
12/30/2025
Epidemiological, Clinical, and Biomarker Profile of Male Infertility in Morocco: A Retrospective Single-Center Study of 1399 Cases
Henri Hubert Kwizera Tsinda, Modou Mamoune Mbaye, Loïc Koumba, Reine Rolande Ada Edou, Achraf Zakaria, Noureddine Louanjli, Bouchra Ghazi, Fatima Maachi, Hakima
IRIFIV Research
07/14/2009
Mutations in the protamine locus: Association with spermatogenic failure?
L. Imken, H. Rouba, B. El Houate, N. Louanjli, A. Barakat, A. Chafik, K. McElreavey

12 Next

prêt à démarrer votre projet parental?

Nous sommes ici pour vous offrir des renseignements, des conseils, du soutien et de la compréhension.

prendre rendez-vous

prêt à démarrer votre projet parental ?

Nous sommes ici pour vous offrir des renseignements, des conseils, du soutien et de la compréhension.

prendre rendez-vous

Nos Services

À PROPOS

RESOURCES

Fondé en 1992, avec plus de 34 ans d’expérience et des milliers de bébés nés, IRIFIV Casablanca est le premier centre structuré marocain de procréation médicalement assistée et l’un des centres de fertilité et FIV les plus reconnus à Casablanca et au Maroc. La clinique est spécialisée dans tous les cas d’infertilité, y compris les cas complexes. Nous utilisons des technologies et équipements de dernière génération, dont certains uniques au Maroc. IRIFIV et le laboratoire Labomac constituent un terrain de stage pratique en FIV dans le cadre du Diplôme Universitaire de Biologie et Génétique de la Reproduction de l’Université Mohammed VI des Sciences de la Santé, et sont actifs en recherche, formation médicale et collaborations internationales.

+212 5 2294 9772

info@irifiv.com

13, place du Nid d’Iris, Quartier Racine, Casablanca, 20000

PATIENTS INTERNATIONAUX

ARTICLES RÉCENTS

IRIFIV is a multidisciplinary fertility center in Casablanca, Morocco, specializing in IVF and ICSI. Since 1992, over 32 years of expertise and thousands of successful births demonstrate our commitment. We offer all techniques to evaluate and treat infertility with a personalized approach, multidisciplinary team, latest technology, complementary therapies, scientific research, teaching, professional development, and international collaboration.

Le 1er Centre multidisciplinaire structuré d’Assistance Médicale à la Procréation (AMP) au Maroc

Fondé en 1992, avec plus de 34 ans d’expérience et des milliers de bébés nés, IRIFIV Casablanca est le premier centre structuré marocain de procréation médicalement assistée et l’un des centres de fertilité et FIV les plus reconnus à Casablanca et au Maroc. La clinique est spécialisée dans tous les cas d’infertilité, y compris les cas complexes. Nous utilisons des technologies et équipements de dernière génération, dont certains uniques au Maroc. IRIFIV et le laboratoire Labomac constituent un terrain de stage pratique en FIV dans le cadre du Diplôme Universitaire de Biologie et Génétique de la Reproduction de l’Université Mohammed VI des Sciences de la Santé, et sont actifs en recherche, formation médicale et collaborations internationales.

+212 5 22 94 97 72

e-mail IRIFIV

IRIFIV Center

Nouveau!

Découvrez notre programme de recherche continue et consultez plus de 65 publications scientifiques de l’IRIFIV sur la fertilité et la médecine de la reproduction

Le Premier Centre de PMA au Maroc

EVALUATION DE LA FERTILITE

REPRODUCTION ASSISTÉE

PRÉSERVATION DE LA FERTILITÉ

TECHNIQUES

L’INFERTILITÉ MASCULINE

TESTS GÉNÉTIQUES

CHIRURGIE DE LA FERTILITÉ

SOUTIEN AUX PATIENTS

Nouveau!

Découvrez notre programme de recherche continue et consultez plus de 65 publications scientifiques de l’IRIFIV sur la fertilité et la médecine de la reproduction

Prêt à démarrer votre projet d‘enfant? Appelez-nous au +212 5 22 94 26 26

INFORMATIONS PARTIQUES

PATIENT INTERNATIONAUX

Planifier Votre Grossesse

RESOURCES

EXPÉRIENCES DE PATIENTS

IRIFIV Research

Evaluation of In vitro Vitamin D Supplementation and its Impact on Sperm Quality: A Controlled Experimental Study

A Fertility Research Publication from IRIFIV – Centre de Fertilité Casablanca, Maroc

Authors

Noureddine Louanjli, Chaimaa Hilali, Ez-Zahidy Douae, Chafika Nadifi, Houda Benrahma, Nouama Bouanani, Kettani Halabi Mohamed, Moncef Benkhalifa, Noureddine Louanjli, Fatima Azzahra Lahlou

Journal / Date

Journal of Human Reproductive Sciences

09/09/2025

Introduction & Clinical Relevance:

Abstract:

Keywords:

Vitamin D, male infertility, sperm quality, sperm motility, sperm DNA fragmentation, in vitro study, asthenozoospermia, assisted reproduction, IVF male factor

IRIFIV Research

Evaluation of In vitro Vitamin D Supplementation and its Impact on Sperm Quality: A Controlled Experimental Study

A Fertility Research Publication from IRIFIV – Centre de Fertilité Casablanca, Maroc

Authors

Noureddine Louanjli, Chaimaa Hilali, Ez-Zahidy Douae, Chafika Nadifi, Houda Benrahma, Nouama Bouanani, Kettani Halabi Mohamed, Moncef Benkhalifa, Noureddine Louanjli, Fatima Azzahra Lahlou

Journal / Date

Journal of Human Reproductive Sciences

09/09/2025

Introduction & Clinical Relevance:

Abstract:

Keywords:

Vitamin D, male infertility, sperm quality, sperm motility, sperm DNA fragmentation, in vitro study, asthenozoospermia, assisted reproduction, IVF male factor

À propos du département de recherche scientifique de l’IRIFIV

À propos du département de recherche scientifique de l’IRIFIV

Vous aimerez aussi

IRIFIV Research

Acomprehensive review about sperm-oocyte interactions and key activation factors of fertilization in mammals

IRIFIV Research

Epidemiological, Clinical, and Biomarker Profile of Male Infertility in Morocco: A Retrospective Single-Center Study of 1399 Cases

IRIFIV Research

Mutations in the protamine locus: Association with spermatogenic failure?

prêt à démarrer votre projet parental?

prêt à démarrer votre projet parental ?

Nos Services

À PROPOS

RESOURCES

PATIENTS INTERNATIONAUX

ARTICLES RÉCENTS

Le 1er Centre multidisciplinaire structuré d’Assistance Médicale à la Procréation (AMP) au Maroc

Comment aimeriez-vous commencer aujourd’hui ?

+212 5 22 94 97 72

+212 5 22 94 97 72